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Adult X-gal staining

Ghysen Alain aghysen at ulb.ac.be
Fri Jul 5 11:35:30 EST 1996


"Does anyone know how to do wholemount X-gal staining on
newly eclosed adult flies?"

Very young flies (or pupae once extracted from the puparium) ) can
be X-gal labelled by using the same protocol as for embryos, including
the heptane-fixative styep which presumably removes the waxy layer
that prevents penetration of the X-gal chemicals through the adult
cuticle. The protocol we use is described in Ghysen and O'Kane
Development 105, 35-52.




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