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Any Qiagen RNeasy users out there?

Jean Leloup pomme at acpub.duke.edu
Mon Jan 29 17:11:27 EST 1996

In article <jreiss-2401961804280001 at pelican.biology.emory.edu>,
jreiss at biology.emory.edu (Jonathan A. Reiss) wrote:

> I am interested in using this system for production of small amounts of
> total rna for rt-pcr experiments on 100-200 embryos.  it's obvious that
> the key step is getting a good homogenate. 
> help/suggestions/caveats?
> thanks.
> jonathan reiss
> jreiss at biology.emory.edu

Watch out, chloride inhibits reverse transcriptase (see in Maniatis).

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