Help !!! I am having all kinds of problem with southern blot
hybridization. I am new on Drosophila, and I would like to know some
basic things as how much DNA in general one load in a slot for
hybridizing with a single copy gene. The specific activity of the probes
used and so on...please, anybody with experience in hybridization tellme
about your experience, because I am driving crazy with frustrating
results on such basic step !!!