I think the ³vertebrate people downstairs² give what would normaly be
considered good advice, although I think there are numerous alternatives,
such as BSA or OVA. I think you should affinity purify your positive
antiserum, using your peptide conjugated to a carrier that is different
from your immunogen, in order to get a truly monospecific (as monospecific
as they get) antiserum. So, I think the carrier you use may not matter
from a crossreactivity point of view.
I hope this is helpful. Please contact me if there is anything else in
this matter you would like to discuss.
In article <cok-1711951941090001 at 188.8.131.52>, cok at mole.bio.cam.ac.uk
(Cahir O'Kane) wrote:
When raising antibodies to peptides, does anyone have any recommendations
for or against particular proteins to which one can couple the peptide?
The vertebrate people downstairs here recommend keyhole limpet hemocyanin
- but given that this is also an arthropod, is there any potential problem
with crossreactivity to a fly homolog of KLH?
Cahir O'Kane, Dept of Genetics, Downing St., Cambridge, CB2 3EH, UK
cok at mole.bio.cam.ac.uk
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