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PCR based P-element rescue

Bill Engels wrengels at facstaff.wisc.edu
Wed May 31 08:57:21 EST 1995

In article <3qi1kc$nql at comet.EMBL-Heidelberg.DE>, Martin Zeidler
<zeidler at embl-heidelberg.DE> wrote:

> Does anyone have details of the primer seaquence and annealing temps for 
> the rescue of genomic DNA flanking a non-rescuable P-element insertion. 

31 May 1995

I think the details you want are in Gloor et al., (1993, Genetics 135:81-95).

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William R. Engels                     :     WREngels at facstaff.wisc.edu
Genetics Department, 445 Henry Mall   :         office: (608) 263-2213
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