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PCR based P-element rescue

Martin Zeidler zeidler at embl-heidelberg.DE
Wed May 31 10:22:52 EST 1995

Does anyone have details of the primer seaquence and annealing temps for 
the rescue of genomic DNA flanking a non-rescuable P-element insertion. 
I assume such a protocol would involve the digestion and 
re-circularisation of genomic DNA with a selection of restriction 
enzymes followed by PCR amplification of the circularised DNA containing 
the P-element LTR sequences and its neighbouring genomic region. 
Any tips or information ?


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