Does anyone have details of the primer seaquence and annealing temps for
the rescue of genomic DNA flanking a non-rescuable P-element insertion.
I assume such a protocol would involve the digestion and
re-circularisation of genomic DNA with a selection of restriction
enzymes followed by PCR amplification of the circularised DNA containing
the P-element LTR sequences and its neighbouring genomic region.
Any tips or information ?