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Blocking microsatellite hybridization in situ

Mark Q. Benedict MQB0 at CIDDPD2.EM.CDC.GOV
Thu May 18 07:56:14 EST 1995

Fellow Users,
We are hybridizing fairly short PCR-amplified microsatellite-containing 
DNAs to polytene chromosomes of the malaria mosquito Anopheles gambiae.  We 
would like to block the secondary hybridization sites with microsatellite 
oligos.  Could you suggest a reliable method for doing so?  (We use 
biotin-labeled DNAs.)

Mark Q. Benedict
Emory U./Centers for Disease Control and Prevention

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