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Summary: preventing fly food falls.

bernhardt at bscr.uga.edu bernhardt at bscr.uga.edu
Fri May 19 13:31:13 EST 1995


	Here are some ideas I received for making fly food less likely to
collapse when a culture bottle is inverted.  Many thanks to:

Greg Fitzgerald <gregory.fitzgerald at yale.edu>
Harald Vaessin <hvaessin at magnus.acs.ohio-state.edu>
Dan Rohwer-Nutter <dlrohwer at facstaff.wisc.edu>
LarkClaass <larkclaass at aol.com> 
Charlie Wright <cw117 at mole.bio.cam.ac.uk>
Adrian Richard Gareth Pugh <argp at st-andrews.ac.uk>

	1. Add one or two sterile Kim-Wipes to act as a wick for additional 
moisture.
	2. Boil the fly food longer - this will kill all the yeast in the
media.
	3. Design your own media bottle to solve the problem.  (Ask Dan!)
	4. Tear off a piece from a sheet of cotton large enough to cover
the surface of the media.  Poke it into the goo around the edges.
	5. Use a glass Erlenmeyer flask shaped bottle - this makes it
harder for the food to fall out of position.
	6. Use more media per bottle.
	7. *Maybe* add more agar.  Be careful - females may not lay eggs
into such media.
--
Matt Bernhardt
University of Georgia                Fools multiply folly.
bernhardt at bscr.uga.edu 



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