IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

CO2 Anesthetization

Kenneth D. Bergman kbergman at keene.edu
Wed May 10 15:21:20 EST 1995


Drosophila Netters,

I'd like to interrupt the usual dialogue about enhancer-trapping and codon
bias with a more elementary inquiry concerning basic fly handling.

Having used ether and (with mixed results) Carolina's Flynap
(triethylamine) for Drosophila anesthesia in an introductory (sophomore)
Genetics lab, I'd like to explore the use of CO2 as a fly anesthetic.  (CO2
use might be more compatible with other functions in a multiple-use lab
facility).

I don't have immediate access to Drosophila methods books or handbooks and
need to gather information rather quickly, in order to facilitate
discussions on lab space-use with an architect.  Hence, I'd appreciate any
advice from those of you who have experience in the use of CO2 in
educational (versus research) settings.
I used CO2 in grad school for anesthesia during insect surgery, but I
haven't had much experience applying it to Drosophila.

My questions:
1 What are the most convenient forms of delivery of CO2?  Via hoses from
gas cannisters?  Dry ice?    What are the advantages and disadvantages of
each?

2 What are the risks of CO2 use in terms of fly mortality?  Sterility?  

3.Do the flies remain immobile only while immersed in gaseous CO2, or does
the exposure induce longer-lasting anesthesia?  (And if so, how
long-lasting is the effect?)

4. If it is necessary to keep the flies under CO2 while sorting on a
microscope stage, how does one manage this?  What kind of arrangements are
necessary?

I'd appreciate any advice, preferably fairly explicit and detailed.

Thanks.  

***************************************************
*  Dr. Kenneth D. Bergman       Prof. of Biology  *
*  Keene State College           (603) 358-2580   *
*  229 Main Street            kbergman at keene.edu  *
*  Keene, NH  (USA)  03435-2001                   *
***************************************************





More information about the Dros mailing list

Send comments to us at biosci-help [At] net.bio.net