Hi there I have been using Ashburner's protocol for extracting high M.W.
DNA from adult flies. Recently I have made several batches of DNA with
good purity (OD260/280 ~1.7-1.8) but which seem to restrict very slowly.
Even overnight the digestion is minimal. Has anybody out there got a
protocol that is as quick as Ashburners, but relatively hassle free?
The DNA is for genomic southerns so as gentle method as possible is
Thanks in advance, Richard M. Badge.