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Help- genomic DNA

pdxrmb at vaxi.nott.ac.uk pdxrmb at vaxi.nott.ac.uk
Thu Mar 2 11:21:09 EST 1995

Hi there I have been using Ashburner's protocol for extracting high M.W.
DNA from adult flies. Recently I have made several batches of DNA with 
good purity (OD260/280 ~1.7-1.8) but which seem to restrict very slowly.
Even overnight the digestion is minimal. Has anybody out there got a 
protocol that is as quick as Ashburners, but relatively hassle free?
The DNA is for genomic southerns so as gentle method as possible is 
Thanks in advance, Richard M. Badge.

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