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vitelline removal

CLANCY David gendjc at LUXOR.LATROBE.EDU.AU
Tue Jun 27 11:11:46 EST 1995


Hi all,
	I'm having fearful trouble removing the vitelline membrane from 
D. serrata (melanogaster group) embryos. Simulans is fine. I'm shaking in 
a 50/50 soln of n-heptane and 80% ethanol. If I use 100% methanol it 
makes no difference. I get very few falling out of the organic layer.
Any suggestions?

thanks

David Clancy
genetics
la Trobe Uni.
Melbourne, Australia




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