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transient transfections in embryos

Susan Lindquist s-lindquist at uchicago.edu
Mon Jun 5 11:06:43 EST 1995

We would like to perform some expression assays with embryos microinjected with various hsp plasmids.  If anyone out there is don't transient transfections we'd appreciate some advice. For example, how long does it take for embryos to show expression after microinjection, asuming you have a good promoter.  Do they require time to recover from the injection.

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