Does anybody had any success with Drosophila embryo transformation using
the plasmid purified on Qiagen Coloumns (Qiagen plasmid purification kit)?
The plasmid prepared by this method looks better quality than CsCl
gradient purification, at least on EtBr gel.
And also, does any body know the effect of EtBr contamination with the
plasmid (after CsCl purification) has any effect on the transformations?
I greatly appreciate netters comments and suggestions.
Thanks in advance