On 14 Feb 1995, Lynn Manseau wrote:
> Have people successfully used Quiagen prepped DNA for fly transformation?
> Do your transformation frequencies drop?
> Also, are there any hints for cosmid transformation into flies?
>> Lynn Manseau
I have frequently used DNA prepped on Qiagen columns for transformation. I
found that overloading the columns would give DNA that looked pretty clean
(i.e., O.D., cut with restriction enzymes, etc.) but wouldn't give
transformants when injected. As a rule of thumb I used 100 ml overnight
cultures in TB broth (see the new Maniatis) on the Qiagen-tip 500
columns. That has not failed for ~10 constructs. Better yet, it has
also worked for other people I have told.