I am having a problem with my SL-2 culture and was wondering if anyone
has ever run into similar problems.
I am growing cells in spinner flask cultures, using Shields and Sang's M3
media and 10% FCS. Originally I had no problem getting the cells up to a
good concentration and then all of a sudden, they stopped growing. They
are not dead (I have done viability assays) yet they remain at a
concentration which is only one third to half what I used to get them to.
I have tried adding more serum, and glutamine to my media.
I'm not sure what else to try.
Any help would be greatly appreciated.
University Of Toronto
nwinegar at credit.erin.utoronto.ca