IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

SL-2 Cell culture

Neil Winegarden nwinegar at tuzo.erin
Mon Sep 26 18:35:47 EST 1994

I am having a problem with my SL-2 culture and was wondering if anyone 
has ever run into similar problems.

I am growing cells in spinner flask cultures, using Shields and Sang's M3 
media and 10% FCS.  Originally I had no problem getting the cells up to a 
good concentration and then all of a sudden, they stopped growing.  They 
are not dead (I have done viability assays) yet they remain at a 
concentration which is only one third to half what I used to get them to.  

I have tried adding more serum, and glutamine to my media.

I'm not sure what else to try.

Any help would be greatly appreciated.


Neil Winegarden
University Of Toronto
nwinegar at credit.erin.utoronto.ca

More information about the Dros mailing list

Send comments to us at biosci-help [At] net.bio.net