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myc epitope tag in flies

Jay Hirsh jh6u at virginia.edu
Sun Oct 2 15:05:00 EST 1994


Hello,

I am interested in "tagging" a protein with an 11 amino acid c-myc epitope
tag to be expressed in flies.  I have found a few examples in which proteins
have been transgenically expressed and immunostained using a myc tag
specific monoclonal Ab, however all of these genes were expressed behind a
heat shock promoter.   

Do any of you know of a case in which someone has tagged a gene that is
driven by its endogenous promoter and sucessfully immunostained tissues from
the transgenic flies?  If so, could you please e-mail me at: meller at virginia.edu

Thanks in advance,

Margaret Meller
Univ. of Virginia
Dept of Biology



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