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stocks to be discarded

Kathy Matthews matthewk at FLY.BIO.INDIANA.EDU
Mon Nov 7 19:50:06 EST 1994

The Bloomington Stock Center is culling the transposon insertion collection 
to allow us to handle insertion stocks that are better characterized and/or 
more versatile.  Stocks were chosen for elimination for one or more of the
following reasons. 

1. The insertion is unlocalized.  The only unlocalized lines being
retained are those associated with a sterile phenotype, and those with
tissue-specific lacZ expression patterns that serve as broadly useful
markers of specific structures or tissue types.  Sets of unlocalized
inserts that express lacZ in a given tissue in a variety of patterns are
no longer collected by the center.

2. The site of insertion is poorly localized, in doubt, or multiple
insertions are present. 

3. The insertion is localized to a region that is well represented by
other insertions that are being retained.  Stocks from the Berkeley
Drosophila Genome Project are given first priority, followed by
enhancer-trap insertions marked with one of the mini-w's and neo-
marked inserts, followed by w[+]-marked non-enhancer-trap lines,
with ry[+] non-enhancer-trap insertions having lowest priority. 

4. The insertion is unstable.  

5. The insertion is allelic to another insertion being retained.  

6. The identity of the transposon construct is unknown. 

The complete list of stocks slated for removal has been posted on
FlyBase in the file stocks/stock-centers/bloomington/discards.rpt2.
If you want to receive any of these stocks before they are discarded,
place your request by stock number by December 7, 1994.  The original 
donors of stocks to be discarded will be notified directly.

--  Kathy

Department of Biology		MATTHEWK at INDIANA.EDU
Indiana University              MATTHEWK at IUBACS    
Bloomington, IN  47405-6801     812-855-5782

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