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** Help with AO staining for drosophila salivary glands

Sinisac Mike sinisac at server.uwindsor.ca
Wed Aug 10 22:08:03 EST 1994

I would like to know if anyone has a staining protocol for
staining the salivary glands of drosophila with Acridine Orange.
I am interested in viewing the differentiation between the RNA
(chromosome puffs) and DNA portions of the polytene chromosomes 
located in the salivary glands of another class of diptera. 
I've tried several things already and am have been unable 
to come up with anything really good yet. Any help would be 
much appreciated.

sinisac at server.uwindsor.ca

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