I am about to try P-element mediated transformation for the first time. I
am using a pCaSpeR construct with the hsp70 promoter and 3'trailer. The
cDNA I cloned in has about 560 bp upstream of the ATG and it has 11 C's at
the 5'most end (artifact of the original cDNA cloning process). Is there
any reason I should get rid of either the polyC region or the whole
upstream 560bp in terms of getting good expression of the transgene? I'd
appreciate any advice.
kcrossgr at mail.sas.upenn.edu