We do not work with Drosophila, but are interested in
complementing some of the Drosophila mutants with a gene from
another eukaryotic organism.
Is anyone aware of experiments (published or not) whereby stable
transformation and expression of a heterologous eukaryotic gene
was shown to complement a Drosophila mutant deficient in an
We are interested in any suggests from Drosophila researchers
which may help us avoid common or previously encountered problems
with similar experiments.
As an aside: Would most people consider such an experiment
(if complementation works) strong enough evidence to assign a
function to the heterologous gene? ie. How strict is
Thanks in advance for any advise and comments
University of Calgary
sjszarka at acs.ucalgary.ca