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Conjugate/Block Buffers

Leslie Confer lconfer at moose.erie.net
Wed Jan 31 01:30:49 EST 1996


In article <199601291418.OAA11091 at caird.scri.sari.ac.uk>,
   djones at SCRI.SARI.AC.UK (D Jones) wrote:
>I'm presently doing some comparisons between different buffers to 
dilute
>my labelled antibody in, for use in ELISA. So far I have established 
so
>far is that the standard conjugate buffer that we use here (2% PVP, 
0.2%
>Ovalbumin in PBS-T) can be improved upon with the addition  of
>various other proteins such as BSA and Casein. I was wondering 
whether
>anyone was using a buffer that they have found to be better than
>anything else they have tried, or does anyone know any commercial
>suppliers of conjugate/block buffers that they have found to be any 
good.
>
>Thanks 
>David
>
>

Can you give more information about what type
of conjugate you are trying to stabilize, and
what your current problems are.  With more
info, I may be able to reach into my bag of
tricks and possibly help you out.

Best regards,
Les Confer

lconfer at moose.erie.net



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