JO DANIELS wrote (with liberal reformatting):
I HAVE POOLED 100 INDIVIDUALS TOGETHER AND TYPED A POLYMORPHIC MARKER
IN THAT POOL SUCH THAT:
- ALL ALLELES PRESENT ARE REPRESENTED BY A PEAK
- ONE (peak) PER ALLELE
- THE HEIGHT OF THE PEAK DEPENDS ON THE FREQUENCY OF THE ALLELE.
I DO NOT KNOW THE NUMBER OR FREQUENCY OF ALLELES IN MY POOL - THAT IS
WHAT I AM TRYING TO EVALUATE.
THE PROBLEM LIES IN THAT EACH ALLELE PRODUCES ARTIFACTS THAT ARE
DEPENDENT ON THE FREQUENCY AND NUMBER OF OTHER ALLELES IN THAT POOL.
HOW CAN I GET RID OF ARTIFACT...
It seems to me that you need to provide more information on the nature
of the artifact(s) that you are seeing. Why pool instead of just