I am doing research on the heat inactivation of proteases.
Around the denaturation temperature autodigestion=autoproteolysis occurs, probably intermolecular.
Now I want to model this inactivation numerically, using the following formulas:
native < - > unfolded
native + unfolded < -- > native/unfolded complex -- > native + inactivated enzyme
native + substrate (other protein) < -- > native enzyme substrate complex -- > native enzyme + products
(Michaelis-Menten Enzyme kinetics)
? Does anyone know articles/research on the kinetics of autoproteolysis reactions?
? Does anyone know a program for making these simulations?
? Can I use MM kinetics for the autodigestion reaction?
Wageningen Agricultural Universty
erix.schokker at zuivel.lmt.wau.nl