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[Chlamydomonas] Chlamy toxin

Ben Engel via chlamy%40net.bio.net (by bdengel from gmail.com)
Mon Oct 27 23:32:44 EST 2014


Hi Simrat,

I have never heard of flying chlamy, so either your starting culture was contaminated (that would be my guess) or you have contaminated media or flasks.  

I don't know anything about growing HP, but can it be grown on solid media (in a Petri dish)?  If so, I would recommend streaking very dilute culture onto a plate so that single clonal colonies grow up.  Then you could pick HP colonies away from any contaminating chlamy.

If you still get contamination after that, I would suspect your media or flasks.  And I would give everything a good autoclaving.

Good luck!
Ben




> On Oct 27, 2014, at 3:31 PM, "KAUR, SIMRAT" <S.KAUR1 from nuigalway.ie> wrote:
> 
> Hi Chlamy researchers!
> 
> 
> 
> I visited the Chlamy connection website to connect to the Chlamy research community.
> 
> 
> I am working on Haematococcus pluvialis (HP) cultivation in an open PBR system where I  frequently encounter the problem of chlamy contamination. I have read that Chlamy release fatty acid like toxin which inhibit the growth of HP. I tried formalin to kill motile chlamy cells and have been successful but anticipate that this treatment is affecting HP as well.
> 
> 
> Can you suggest ways in which I can control chlamy contamination in HP cultures.
> 
> Another question is how does chlamy get into HP culture? Is it air borne or water borne or was in the stock culture?
> 
> I received the HP stock culture from SSCAP which was free of any chlamy cells (or i was unable to detect).
> 
> 
> Any suggestions would be highly appreciated.
> 
> ?Many Thanks!
> 
> 
> 
> Regards
> Simrat Kaur
> PhD Scholar
> Botany & Plant Science
> NUIG
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