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[Chlamydomonas] Markers for Chlamydomonas reinhardtii gametes?

Hanna Koch via chlamy%40net.bio.net (by 16hkoch16 from gmail.com)
Thu Jan 9 10:53:14 EST 2014


Hello Chlamy group,

Does anyone have a suggestion for some type of marker (dye, stain,
antibody, etc...) to distinguish Chlamydomonas reinhardtii gametes from
vegetative cells that are suspended together in liquid culture (without
having to do a visual check - and without having to add cells of the
opposite mating type to produce a mating reaction)?
Or another somewhat fast & simple method for distinguishing the two, that
would accomodate a high-throughput project?

Thank you,

Hanna Koch
PhD Student
Max Planck Institute for Evolutionary Biology
Evolutionary Ecology & Community Dynamics
Plon, Germany


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