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[Chlamydomonas] Liquid culture growth

Heloisa Ciol via chlamy%40net.bio.net (by helociol from gmail.com)
Fri Sep 27 09:22:38 EST 2013


Dear ones,

I'm reaching for you in hope you can help me.

I'm working with *Chlamydomonas reinhardtii* strain CC-4351 cw15 arg7-8 mt+
[Matagne 325] and we've been facing some growth problems in liquid culture.

We've been harvesting the alga in TAP medium supplemented with Arginine,
and it seems the culture does not reach the deep green colour expected
after 4 or 5 days of growth (or even more - we've tried). Also the culture
seems to be more viscous and "milky" then before.

We looked for contaminants in Birght Microscopy and some samples were even
prepared to electronic microscopic, but we did not notice any contaminant
within the Chlamydomonas cells.

We suppose there might be some problem with the media we are harvesting the
alga, but don't know how to overcome this.

Do you have any suggestion of what it could be? Has someone faced the same
problems brefore and could lend some help?

(I have pictures of the liquid culture and some microscopy photos, if it
would help).

I appreciate your help.

Best regards,

-- 
Heloísa Ciol


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