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[Chlamydomonas] how to limit growth

Christian Jebsen via chlamy%40net.bio.net (by jebsen from rz.uni-leipzig.de)
Wed Jan 7 04:38:15 EST 2009


A turbidostat will be the right culturing method for you, this would be 
a technical way to limit the cell density. A change of the culture media 
or rather the nutrient supply to limit cell density would always effect 
the motility and the health status of the cells.  The cells could loose 
their motility and would reduce their metabolic turnover.

chlamy-request from oat.bio.indiana.edu wrote:
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>    1. how to limit growth? (Seppe Kuehn)
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> Date: Mon, 5 Jan 2009 15:03:55 -0500
> From: Seppe Kuehn <skuehn from mail.rockefeller.edu>
> Subject: [Chlamydomonas] how to limit growth?
> To: chlamy from magpie.bio.indiana.edu
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> We are looking for a protocol for growing Chlamydomonas Reinhardtii  
> under phototrophic conditions where the final cell density is limited  
> to ~10^5 cells/mL.  We would like to do this without adversely  
> effecting motility, metabolism, or overall health of the culture.     
> We are currently working with the UTEX 2244 (mt+) strain (also known  
> as CC-125) but are open to using other strains if necessary.  Thus  
> far Sager's medium has worked best for our application so a simple  
> modification to this medium that limits final cell density would be  
> ideal.  Thank you! 
>
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-- 
Christian Jebsen (Dipl.-Biol.)
University of Leipzig
Department of Plant Physiology
Johannisallee 21, 04103 Leipzig
Phone: #49-341-9736876   Fax:#49-341-9736899
jebsen from rz.uni-leipzig.de, http://www.uni-leipzig.de/~pflaphys



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