I am growing 25B1 strain (Newman et al. 1991) in acetate medium with 130 rpm and 200 rpm in dark. However, it does not attain OD 0.6 or above in 5 -10 days, whereas wild type cells like 2137+ or cw15- reach to ca. OD 0.6 in similar conditions. Of course light is provided for these two strains. (ca. 15- 20 micromoles).
What are the best conditions required to increase the cell density of 25B1 strain.