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getting good flagellation?

Chris Jones kuritaro at earthlink.net
Wed Apr 10 21:19:01 EST 2002

I'm trying to grow Chlamydomonas for an undergraduate lab looking at
flagellar regeneration. Trouble is, although growth is good,
flagellation isn't great. It looks like at most 30% of my cells are
flagellated (judging from motility).

Is this typical, or is there something I could do to increase my
proportion of swimmers? I'm growing in M1 medium (as per Sager and
Granick) with gentle shaking and 2 x 15 W fluorescent bulbs about 6
inches away. Does anyone have any suggestions? Thanks,

Chris Jones

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