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Chlamy cultuture in darkness

Tue Mar 16 16:09:07 EST 1999

I am going to isolate mitochondrial mutants and revertants of Chlamydomonas
reinhardtii. We have to bring them up in darkness to select mitochondrial
revertant clone from deffecient clone because they can grow without
functional mitochonria in light condition. The problem is, in darkness,
Chlamydomonas reinhardtii CW15 take 4 weeks to grow up as colonies on
agarose plates of TAP medium. It would be very appreciated if someone could
tell me a method/medium to grow them faster in the darkness.
Kazuo Watanabe

Ohama Laboratory
Biohistory Research Hall, 1-1 Murasaki, Takatsuki,
Osaka 569-11,   Japan
Tel: 0726-81-9761
Fax: 0726-81-9757
e-mail: watanabe at ims.brh.co.jp

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