I think it convenient to measure the rate of glycolate excretion in the
presence of 1 or 2 mM aminooxyacetic acid, at least for the wild type
strains of Chlamydomonas reindardtii. For details, check papers such
Tolbert, N.E., Harrison, M. and Selph, N. (1983) Plant Physiol. 72:
Yokota, A. and Kitaoka, S. (1987) Planta 170:181-189.
Hope it helps.
> I have some undergrads who want a simple method for measuring the
> rate of photorespiration. I remember reading somewhere about the
> use of phenylhydrazine to measure the glyoxylate produced in
> photorespiration. Does anyone have further details of this method,
> or does anyone know of a "better" method which could be used.
Kensaku Suzuki | E-mail: suzuki at affrc.go.jp
Plant Eco-Physiol Lab | suzuki at tnaes.affrc.go.jp
Tohoku Natl Agric Expt Stn | Phone: +81 19 643 3467
Morioka 020-0123, Japan | FAX: +81 19 641 7794