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staining live vs. dead cells

Malcolm Campbell macampbell at davidson.edu
Tue Sep 15 13:31:31 EST 1998


I am trying to develop a variation on the teaching lab where students
measure the rate of flagella regeneration. We use pf14 cells so that the
flagella will be straight. I would like to use a vital dye like Nile Blue
to distinguish between dead cells that will never regenerate flagella and
live cells that might be able to regenerate flagella. The problem has been
that since we cannot tell live cells from dead, the students will record
zero lengths at every time point which gives them too large of error bars.
Do you know a way to distinguish dead and live cells?
Thanks,

A. Malcolm Campbell
Biology Department
Davidson College
P. O. Box 1719 (US mail) or 217 Concord Rd (shipping)
Davidson, NC     28036
phone:  (704) 892-2692
fax: (704) 892-2512
email: macampbell at davidson.edu
www: http://www.bio.davidson.edu/






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