Two Postdoctoral Positions
Molecular Mechanisms of Gene Silencing
Postdoctoral positions are available immediately to study the molecular
mechanisms responsible for epigenetic transcriptional inactivation. We
have recently demonstrated that two processes, namely chromatin/nuclear
domains and DNA methylation, might cause the silencing of foreign
transgenes in the unicellular green alga Chlamydomonas reinhardtii (Plant
Cell 9: 925-945 ; Genetics 145: 97-110 ). To test these
hypotheses, our specific research objectives are directed at identifying
and characterizing some of the gene products responsible for the
inactivation. We are currently isolating tagged mutants defective in
epigenetic silencing; cloning and characterizing several tagged genes
involved in transcriptional repression (homologous genes will be isolated
in Arabidopsis); and mapping the chromosomal location of silenced and
stable transgenic loci.
We expect that these results will contribute to a greater understanding of
epigenetic phenomena in eukaryotes, including higher plants and mammals,
since the molecular mechanisms appear to be evolutionarily conserved. This
knowledge, in turn, might be useful for improving transgenic technology.
Successful candidates will have access to state-of-the-art facilities in
the George W. Beadle Center for Genetics Research and acquire experience in
epigenetics, chromatin, and DNA methylation analyses. A background in
molecular genetics and biochemistry would be desirable.
The project is supported by NSF and a Nebraska Research Initiative and the
positions are fully funded for two years with the possibility of renewal
for a third year dependent on satisfactory progress. Applicants should
send a CV, statement of research experience, and names and addresses
(including e-mail) of three references to: Dr. Heriberto Cerutti, School
of Biological Sciences, University of Nebraska, E211 Beadle Center, P.O.
Box 880666, Lincoln, NE 68588-0666; phone (402) 472-0247; fax (402)
472-8722; e-mail hcerutti at unlserve.unl.edu.