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contamination in Chlamy culture

marcel janssen Marcel.Janssen at ALGEMEEN.PK.WAU.NL
Thu Nov 13 09:42:07 EST 1997


Dear colleagues,

I am trying to maintain a pure chlamy culture, a mt+ wild type strain (CC
1690 or 21 gr). Very frequently cultures appaer to be infected by a very
small micro-organism. Its diameter is less than one tenth of the diamater of
a chlamy cell. The organism is only visible at a magnification of 1000 under
a phase-contrast microscope. The organism is motile and looks to be greenish
in the interior.

1) Does anybody have an idea what kind of organism this is? (a small green
alga, a (photo-autotrophic) bacterium or...)
2) Wil it harm my culture? Or is the organism just living from algal residues
without influencing the algae?
3) How could I get rid of it? the answer of this probably depends on the
answer of question 1.

thanks very much for your time,

sincerely,

Marcel Janssen
Department of Food Science
Food and Bioprocess Engineering Group
Wageningen Agricultural University
P.O.B. 8129
6700 EV Wageningen
The Netherlands

Telephone: +31 (0)317-483396
Telefax: +31 (0)317-482237
E-Mail: Marcel.Janssen at algemeen.pk.wau.nl






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