I wonder if anyone can help me. I am having a few problems making up
bulk media for chlamydomonas reinhardtii. I really need to be able to
make up the media in a block to avoid having to add anything after
autoclaving, apart from my innoculum. I also want to avoid the use of
non-specific compounds such as "yeast extract" or "lab-lemco powder".
This is so I can calculate the osmolarity of the medium from the
molecular weights of the added compounds. In brief the specifications
I require are as follows;
a. "All in one" mix that will not precipitate on autoclaving.
b. Use of only chemically defined ingredients (no yeast extract).
c. "Complete" media for rapid and sustained growth.