IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

zygote DNA

Jim Umen umen at biodec.wustl.edu
Tue Sep 10 14:10:07 EST 1996


Chlamy Netters,

I have been trying to purify  zygote DNA  from an inefficient mating
reaction (%10-20 mating). After ~12 hrs of mating, I add NP-40 to lyse
unmated gametes.  This step works very efficiently.  However, even after
putting the cells on a percoll gradient to remove starch and debris,
all the lysed cell DNA copurifies with the zygote fraction.  I may
try DNAse but am worried that the unmated cell DNA is in an inaccessible
form.  Does anyone have experience or suggestions on improving this
purification protocol?

 Jim Umen                                         ---
 Department of Biology                           /   \
 Campus Box 1229                                /     \
 Washington University                          | @   |
 One Brookings Drive                            \     /
 St. Louis, MO  63130                            \   /
                                                  -V-
                                                  / \
                                                 /   \
 Ph: 314-935-6855                   _-_-_-_-_-_-_     -_-_-_-_-_-_-
 Fax:314-935-5125                                \   /
                                                  \ /
                                                  -v-
                                                 /   \
                                                /     \
                                                |   @ |
                                                \     /
                                                 \   /
                                                  ---






More information about the Chlamy mailing list

Send comments to us at biosci-help [At] net.bio.net