Dear Chlamy Researchers:
I have been having occasional problems with Chlamy DNA (isolated by various
methods, including some commercially available kits, but not by CsCl density
gradient ultracentrifugation): it always restricts okay, but sometimes degrades
slowly in the refrigerator over time, and sometimes gives "muddy" or smeared
Southern blots. Undoubtedly the problem is with variable purity of the DNA.
I know at least a couple of labs are continuing to use CsCl gradient
ultracentrifugation...... but has anyone had good experience getting RELIABLE
DNA preps for Southern blotting from any NON-CsCl method?
I'd be interesting in hearing about the method(s). Thanks very much.
Dept. of Biology
Univ. of Miami
Coral Gables, Florida, U.S.A.