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Heat shock proteins

John A. Berges berges at BNLUX1.BNL.GOV
Mon Oct 30 09:59:08 EST 1995


Hi Folks,

I am currently working with the "chlamydomonas relative" Dunaliella
tertiolecta.  I have been placing the cells under various light and nutrient
stresses and have been watching the appearance of a proteolytic activity.
Last week we had a culture accident in which temperatures rose from 18 to 35
degrees C...and guess what: the proteolytic activity appeared.

I know very little about heat shock proteins, but it strikes me this may be
one.  I have protein samples frozen away for western blotting, and am
looking for sources of very small amounts of antibodies (i.e. enough for a
couple assays) to heat shock proteins, e.g. HSP 70, HSP 25, or others you
folks are aware of.

Can anyone help me out?

Cheers.
________________________________________________________________
      _/_/_/     _/      _/   _/    Dr. John A. Berges
     _/    _/   _/      _/   _/     Oceanic and Atmospheric
    _/    _/   _/_/    _/   _/      Sciences Divison
   _/_/_/     _/ _/   _/   _/       Brookhaven National Lab
  _/     _/  _/   _/ _/   _/        Upton, NY 11973        
 _/     _/  _/    _/_/   _/         Phone: 516-282-4077
_/_/_/_/   _/      _/   _/_/_/_/    Email: berges at BNLUX1.BNL.GOV
________________________________________________________________





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