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Elizabeth Harris chlamy at ACPUB.DUKE.EDU
Tue Jul 11 14:03:53 EST 1995

Heriberto Cerutti, a post-doc in the Boynton-Gillham lab, tells me that
eliminating the final high-speed centrifugation prior to freezing the
autolysin prep seems to result in higher activity.  He thinks perhaps the
enzyme is partly bound to cell debris.  Since freezing at -80C would
presumably kill any surviving Chlamydomonas cells in any case (as everyone
who's tried to preserve Chlamy by freezing can attest!), this step would
seem to be non-essential.  Has anyone else experimented with this?

Elizabeth Harris
chlamy at acpub.duke.edu

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