>From: HAROLD HOOPS <HOOPS at UNO.CC.GENESEO.EDU>
>I teach an undergraduate cell biology laboratory and am trying to think
>up some new "investigative labs" to add to the mix. While attending the
>Chlamydomonas meetings, some of the papers reminded me of the concept of
>dikaryon rescue of the motile phenotype. It struck me that this could
>be the basis of an interesting laboratory.
>>My tentative plan would be to have two different pf mutants (of different
>mating type) capable of complementing each other. After mating one should
>see restoration of motility of each flagellar pair. With the proper leading
>questions, one should be able to get the students to figure out the
>general basis for the restoration of motility.
>>It would be most useful to have 2 easily scoreable but different
>mutant phenotypes. If necessary we could cross one or more mutants with
>wild type, but I think that undergraduates would be able to score absence
>vs. presence of motility in the gametes and zygotes more easily than they
>could tell if 2 or 4 flagella in a zygote were motile. It would also be nice
>to have two strains that will mate but not complement (plus and minus
>strains of the same pf mutant?).
>>The lab, would lend itself to asking questions dealing with the requirement
>for new message or protein synthesis etc. using inhibitors.
>My questions follow:
>>1) Do you know of any elementary laboratory exercises that follow this
>>2) If not, what would be the best strains to use?
>>3) Is there anything tricky about using these strains of getting dikaryon
>rescue. (I have experience in growing Chlamy, including pf mutants, and in
>mating wild-type cells.)
>> Thank you very much for your help (and your patience). If I do make this
>into a good lab. I would, of course, be willing to share it with anyone who
>1 College circle
>Geneseo, NY 14454
>phone: (716) 245-5378
>e-mail: Hoops at uno.cc.geneseo.edu>>>>Elizabeth Harris
chlamy at acpub.duke.edu