As promised here is a summary of the length of 3' parts of cDNAs:
Accession number Length 3'
X61624 700 bp
J04219 670 bp
M36123 90 bp
X66411 30 bp
X66413 12 bp
I didn't check if all these sequences contained an polyadenylation
signal. I only used the information which I could extract automaticly
with a computerprogram. The mean (if you can say that about some 30
cDNA sequences) is about 300 bp.
Does anyone know why there are so many genomic sequences in the library?
Last year it was about 50% of the total chlamy sequences. Is there a
problem with sequencing cDNAs?
Henk van de Kamer
CHLAMY at SARA.NL