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Labelling with amino acids

Jonathan B. Marder MARDER at agri.huji.ac.il
Wed Jan 5 09:40:04 EST 1994

In article <2gaf3a$acd at mserv1.dl.ac.uk> cyohn at scripps.edu (Christopher Yohn) writes:
>Subject: Labelling with amino acids
>From: cyohn at scripps.edu (Christopher Yohn)
>Date: 4 Jan 1994 01:00:26 -0000

>Does anyone have experience labelling Chlamydomonas in vivo with
>radiolabelled amino acids (i.e. 3H or 14C)?  Will the amino acids be taken
>up from the culture media in sufficient quantity to do a pulse labelling of
>protein synthesis?  Is it necessary to toluene-permeabilize the cells to
>accomplish this?  Any protocols, tips, hints, etc. will be greatly
>appreciated.  Thanks in advance.

I have certainly done this with 35S-Methionine.  Many people get good
protein-specific labelling feeding 35S-Sulphate to sulphate-starved cells.

Jonathan B. Marder                 '
Department of Agricultural Botany  |     Internet: MARDER at AGRI.HUJI.AC.IL
The Hebrew University of Jerusalem | /\/ Bitnet:   MARDER at HUJIAGRI
Faculty of Agriculture             |/  \ Phone:    (08 or +9728) 481918

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