In article <2gaf3a$acd at mserv1.dl.ac.uk> cyohn at scripps.edu (Christopher Yohn) writes:
>Subject: Labelling with amino acids
>From: cyohn at scripps.edu (Christopher Yohn)
>Date: 4 Jan 1994 01:00:26 -0000
>Does anyone have experience labelling Chlamydomonas in vivo with
>radiolabelled amino acids (i.e. 3H or 14C)? Will the amino acids be taken
>up from the culture media in sufficient quantity to do a pulse labelling of
>protein synthesis? Is it necessary to toluene-permeabilize the cells to
>accomplish this? Any protocols, tips, hints, etc. will be greatly
>appreciated. Thanks in advance.
I have certainly done this with 35S-Methionine. Many people get good
protein-specific labelling feeding 35S-Sulphate to sulphate-starved cells.
Jonathan B. Marder '
Department of Agricultural Botany | Internet: MARDER at AGRI.HUJI.AC.IL
The Hebrew University of Jerusalem | /\/ Bitnet: MARDER at HUJIAGRI
Faculty of Agriculture |/ \ Phone: (08 or +9728) 481918