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extracts for footprinting

ellis at WUSTLB.WUSTL.EDU ellis at WUSTLB.WUSTL.EDU
Mon Jul 26 11:37:29 EST 1993


	I would like to know if anyone has a good protocol for whole
cell and/or nuclear crude protein extracts from Chlamy that would be
suitable for use in footprinting or band shift experiments with
NUCLEAR genes.  I have tried adapting a whole cell extract procedure
from soybean literature, but I'm not very enthusiastic about the
results so far.  Since isolating nuclei is rather straightforward, I
think nuclear extracts will be more sucessful, but I will still need
to look for some proteins that are likely in the cytoplasm, not the 
nucleus.
	Thanks in advance--

	Lisa Ellis
	ELLIS at WUSTLB.WUSTL.EDU




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