In response to Jim Clack's request for info on enrichment of eyespots from
Chlamydomonas reinhatdtii, I offer up the following reference which purifies
an eyespot fraction from C. reinhardtii and then examines this fraction for G
proteins using binding of radioactive GTPgammaS:
S.N. Korolkov, M.N. Garnovskaya, A.S. Basov, A.S. Chunaev and I.L. Dumler
(1990) The detection and characterization of G-proteins in the eyespot of
Chlamydomonas reinhardtii. FEBS Letters 270: 132-134.
The actual method for obtaining the eyespot enriched fraction is found in
their previous paper:
I.L. Dumler, S.N. Korolkov, M.N. Garnovskaya, M.N. Parfenova and R.N. Etingof
(1989) J. Prot. Chem. 8: 387-389. I have not seen this paper.