I am trying to label isolated flagella with a fluorescent lipid dye to
use in an experiment. I have tried "DiI" C18 (Molecular Probes # D282) by
disolving 10mg/ml in EtOH which was used as a 1000X stock. I added the
dye to the flagella after they were isolated and on ice. The dye did not
label well at all. Does anyone have any suggestions or other dyes that they
have used to label flagella? Please contact me with your suggestions at
"campbell at wustlb.wustl.edu".
Thanks for any help you can offer.