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RT-PCR

"Ambrose R. Kidd III Trey arkidd at students.wisc.edu
Thu Mar 14 23:01:15 EST 2002


I use Trizol reagent (widely available, easy protocol) to prep RNA directly
from 5 - 20 worms.  Then perform a standard RT reaction with oligo dT or a
gene specific primer. PCR on this cDNA on most genes I have tested. Never
tried single worms.

Good Luck.


======================
Ambrose R. Kidd III, "Trey"
arkidd at students.wisc.edu
Dept of Biochemistry
University of Wisconsin - Madison




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