I use Trizol reagent (widely available, easy protocol) to prep RNA directly
from 5 - 20 worms. Then perform a standard RT reaction with oligo dT or a
gene specific primer. PCR on this cDNA on most genes I have tested. Never
tried single worms.
Ambrose R. Kidd III, "Trey"
arkidd at students.wisc.edu
Dept of Biochemistry
University of Wisconsin - Madison