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ATP and longevity in C. elegans

daemon at net.bio.net daemon at net.bio.net
Sun Mar 15 14:52:52 EST 1998

>From The Worm Breeder's Gazette, 1998, v. 15, No. 2, p. 56.

          AN ATTEMPT TO SLOW AGING IN C. elegans.
   Vladimir V. Bakaev, box 45, Novosibirsk, 630107, USSR

The purpose of this study was to investigate the  effect  of
different  concentrations of sodium adenosinetriphosphate in
water solutions on nematode life span.  In  this  experiment
sodium adenosinetriphosphate  was  used in following diluti-
ons: 1:10(3),  1:10(4),  1:10(5), 1:10(6) and 1:10(7). Three
adult animals (3 - 5 days old) were kept in microtitre wells
containing 0,75 ml of liquid medium (with E. coli and witho-
ut  sodium adenosinetriphosphate) during 4 hours,  then they
were discarded and newborn larvae were transferred  in  next
wells (with sodium adenosinetriphosphate in any concentrati-
on) every day (one worm in one well). This investigation was
carried  out  in temperature +21oC and in the darkness.  The
obtained results are presented in the following table.

=C5    Concentration of   =C5     =C5      Longevity (days)      =C5
=C5        sodium         =C5     =DC=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C5 adenosinetriphosphate =C5  n  =C5  mean+/-S.E. =C5   maximal   =C5
=C5       Control         =C5 12  =C5  20,8+/-1,7  =C5      27     =C5
=C5                       =C5     =C5              =C5             =C5
=C5       1:10(3)         =C5 12  =C5   5,6+/-0,2  =C5       6     =C5
=C5                       =C5     =C5              =C5             =C5
=C5       1:10(4)         =C5 12  =C5   6,1+/-0,2  =C5       8     =C5
=C5                       =C5     =C5              =C5             =C5
=C5       1:10(5)         =C5 12  =C5  10,7+/-1,2  =C5      20     =C5
=C5                       =C5     =C5              =C5             =C5
=C5       1:10(6)         =C5 12  =C5  20,7+/-1,3  =C5      27     =C5
=C5                       =C5     =C5              =C5             =C5
=C5       1:10(7)         =C5 12  =C5  20,9+/-1.8  =C5      26     =C5

The nematodes in concentration of sodium  adenosinetriphosp-
hate 1:10(3) were unable to have progeny, then, the concent-
rations 1:10(3) and 1:10(4) were  toxic.  The  concentration
1:10(5) was slightly toxic (the differences between longevi-
ties in concentrations  1:10(3),  1:10(4),  1:10(5)  and  in
control  were  statistically  significant,  P < 0,001 in all
these cases).  The longevities in concentrations 1:10(6) and
1:10(7) and in control were similar (the differences between
longevities in concentrations 1:10(6)  and  1:10(7)  and  in
control were statistically not significant,  P > 0,05 in all
these cases).

Conclusion: If the water solution of  sodium  adenosinetrip-
hosphate  was  applied  to C.  elegans during the whole life
span in above described conditions,  it was not able to pro-
long the life span of these animals.

Acknowledgment: The  author  wishes to express his thanks to
CGC for providing C. elegans (Bristol, N2) and E. coli OP50.

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