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Tue Aug 26 13:38:12 EST 1997

On Tue, 26 Aug 1997 fodoran at ludens.elte.hu wrote:

Dear Liz, Dear Newsgroup,

Although we are going to send these data to the next issue of the
Newsletter, let us share our results with you:


Csaba Bognar(2), Tibor Vellai(1) and Andras Fodor(1),
1. 	Dept. Genetics, Eotvos University, H-1088 Budapest, Muzeum krt. 4/A,
2. 	Bela Johann National Institute of Public Health in Hungary, Department
	of Bacteriology, (OKI), H-1097 Budapest, Gyali ut 2-6, Hungary.

E. coli OP50 is used practically by everybody in the C. elegans Community
as natural food for C.elegans. Our substrain what we use was kindly
provided by Ian Hope in 1995. We have tested its antibiotics sensitivity,
by the paper disc method routinly used the OKI. We used 30 ug/ml of each
antibiotics tested. The filter paper discs are out on the center of the
NGM plate seeded by OP bacteria. An inhibition zone develops, if the
antibiotics is effective against the baccteria under test. Conventionally,
if the diameter oif the inhibition zone is smaller then 2 cm, we conclude
that the bacteria is resistant to the antibiotics tested. We tested 24
antibiotics, as follows:

gentamycin; tobramycin; sumetrolim; chloramphenicol; tetracycline;
ampicyllin; karbenicillin; cefalexine; cefamandol; cefuroxime; amykacin;
netylmycine; azlocilline; mezlocillin; ceftriaxone; cefatoxim;
cefroperazon; ofloxacin; pestloxacin; cyprofloxacim; ceftazidin; cefaclor;
augmentin; and kanamycin. (Some of the names are probably written in very
Hungarian; we promise the correct them in the Newsletter article).

Well, we found that OP50 was completely resistant to all these

I hope these data help Liz and other fellow Colleauges to work with OP50.

Faithfully yours,

Andras and his gang.   

> From:	MX%"ryder at WPI.EDU" 14-AUG-1997 16:32:41.88
> To:	MX%"bionet-news at dl.ac.uk"
> CC:	
> Subj:	Re: OP50 Antibiotic resistance
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> Date: 14 Aug 1997 06:56:36 -0700
> Resent-Date: Thu, 14 Aug 97 15:18:40 UT
> Message-ID: <97814151840.~INN-LWAa00195.bionet-news at dl.ac.uk>
> Resent-Message-ID: <Pine.OSF.3.95q.970814095639.29121B-100000 at bert.WPI.EDU>
> Precedence: list
> From: ryder at WPI.EDU (Elizabeth Ryder)
> Reply-To: ryder at WPI.EDU (Elizabeth Ryder)
> Sender: "bionet.celegans mail newsgroup" <server-daemon at dl.ac.uk>
> To: "bionet.celegans mail newsgroup" <bionet-news at dl.ac.uk>
> Subject: Re: OP50 Antibiotic resistance
> Comments: List problems/queries to <biosci at dl.ac.uk>
> Comments: To mail both the group and netnews send to (celegans at dl.ac.uk)
> X-Article-Number: bionet.celegans Msg # 1646
> X-Listpath: bionet-news
> X-Mailer: MXT V 12.16.1
> All I know is that it's NOT resistant to Amp or Kan.
> Liz Ryder
> Worcester Polytechnic Institute
> Dept. of Biology and Biotechnology
> Worcester, MA  01609
> email:  ryder at wpi.edu
> phone:  508-831-6011
> fax:    508-831-5936
> On Wed, 13 Aug 1997, John M. Hawdon wrote:
> > Hi;
> > 	Anyone off hand know whether OP50 is resistant to any antibiotics?
> > 
> > Thanks
> > 
> > John Hawdon
> > 
> > 

Dr. Andras Fodor			e-mail: fodoran at ludens.elte.hu	
Associate Professor			or:	fodoran at falco.elte.hu
Eotvos University			Phone:	+
Department of Genetics				+ ext. 2348
Budapest, Muzeum krt. 4/A		FAX:	+

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