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3rd generation GFPs in worms?

Lee Honigberg leeh at CGL.UCSF.EDU
Fri Oct 18 00:16:13 EST 1996


Hi,

Has anyone tried GFP with S65T + F64L in worms?
[Cormack et al (Gene 173:33) found this was 35X brighter
than wt GFP (compared to 6X brighter for S65T alone).]

Better yet, has anyone made those changes in any of
the intron-enriched GFP vectors provided by the Fire lab?

Thanks,

Lee Honigberg
Kenyon Lab - Dept. of Biochem.
UC San Francisco
513 Parnassus Ave.
San Francisco, CA 94143-0554





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