Does anybody if and how often transcriptional regulatory elements are
located DOWNSTREAM of the ATG start codon of C.elegans genes ? (i.e. how
"dangerous" is it to fuse GFP to the first exon, thereby potentially
missing regulatory sequences within the first introns...).
Thanks for examples either way !
Oliver
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Oliver Hobert
Harvard Medical School/Department of Genetics
Mass. General Hospital/Department of Mol. Biol.
Ruvkun-Lab
Wellman 8
Boston, MA 02114
Fax: 1-617-726 6893
Tel: 1-617-726 5983
e-mail: hobert at molbio.mgh.harvard.edu
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